{"id":4152,"date":"2014-03-13T12:16:54","date_gmt":"2014-03-13T11:16:54","guid":{"rendered":"http:\/\/histoweb.com\/tecnicas-especiales-2\/"},"modified":"2016-02-22T17:36:57","modified_gmt":"2016-02-22T16:36:57","slug":"special-techniques","status":"publish","type":"page","link":"https:\/\/histoweb.com\/en\/services\/special-techniques\/","title":{"rendered":"Special Techniques"},"content":{"rendered":"<div id='full_slider_1'  class='avia-fullwidth-slider main_color avia-shadow   avia-builder-el-0  el_before_av_textblock  avia-builder-el-first   container_wrap fullsize'  ><div  class='avia-slideshow av-av_slideshow_full-4f3ef5c0d0c5e5a45e27d5f99336c2be avia-slideshow-featured av_slideshow_full avia-slide-slider av-slideshow-ui av-control-default av-slideshow-manual av-loop-once av-loop-manual-endless av-default-height-applied   avia-slideshow-1' data-slideshow-options=\"{&quot;animation&quot;:&quot;slide&quot;,&quot;autoplay&quot;:false,&quot;loop_autoplay&quot;:&quot;once&quot;,&quot;interval&quot;:5,&quot;loop_manual&quot;:&quot;manual-endless&quot;,&quot;autoplay_stopper&quot;:false,&quot;noNavigation&quot;:false,&quot;bg_slider&quot;:false,&quot;keep_padding&quot;:false,&quot;hoverpause&quot;:false,&quot;show_slide_delay&quot;:0}\"  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><ul class='avia-slideshow-inner ' style='padding-bottom: 26.595744680851%;'><li  class='avia-slideshow-slide av-av_slideshow_full-4f3ef5c0d0c5e5a45e27d5f99336c2be__0  av-single-slide slide-1 slide-odd'><div data-rel='slideshow-1' class='avia-slide-wrap '   ><img decoding=\"async\" fetchpriority=\"high\" class=\"wp-image-3086 avia-img-lazy-loading-not-3086\"  src=\"https:\/\/histoweb.com\/wp-content\/uploads\/2014\/11\/tecnolog\u00edas-especiales-pato.jpg\" width=\"940\" height=\"250\" title='tecnolog\u00edas-especiales-pato' alt=''  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/histoweb.com\/wp-content\/uploads\/2014\/11\/tecnolog\u00edas-especiales-pato.jpg 940w, https:\/\/histoweb.com\/wp-content\/uploads\/2014\/11\/tecnolog\u00edas-especiales-pato-300x79.jpg 300w\" sizes=\"(max-width: 940px) 100vw, 940px\" \/><\/div><\/li><\/ul><\/div><\/div><div id='after_full_slider_1'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-4152'><div class='entry-content-wrapper clearfix'>\n<section  class='av_textblock_section av-av_textblock-0366cc7376be6c9e82a3e9cc8987b64f '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h2>SPECIAL TECHNIQUES FOR DIAGNOSIS<\/h2>\n<p><span class=\"Apple-style-span\" style=\"color: #18acb4;\"><b>IMMUNOHISTOCHEMISTRY<\/b><\/span><\/p>\n<p>Immunohistochemical (ICH) techniques are used in diagnostic pathology for the detection of infectious agents in animal tissue samples or to allow a more precise classification of neoplastic diseases. The following antibodies are available:<\/p>\n<table class=\"myTable\">\n<thead>\n<tr>\n<th>Antibody<\/th>\n<th>Antigen<\/th>\n<th>Diagnosis<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>CD3<\/td>\n<td>T Lymphocytes<\/td>\n<td>T-cell Lymphoma<\/td>\n<\/tr>\n<tr>\n<td>CD79a<\/td>\n<td>B Lymphocytes (canine)<\/td>\n<td>B-cell Lymphoma<\/td>\n<\/tr>\n<tr>\n<td>CD45R<\/td>\n<td>B Lymphocytes (feline)<\/td>\n<td>B-cell Lymphoma<\/td>\n<\/tr>\n<tr>\n<td>MHCII<\/td>\n<td>Dendritic cells<\/td>\n<td>Hystiocytic disease<\/td>\n<\/tr>\n<tr>\n<td>PAX5<\/td>\n<td>B Lymphocytes<\/td>\n<td>B-cell Lymphoma<\/td>\n<\/tr>\n<tr>\n<td>CD31<\/td>\n<td>Endothelial cells<\/td>\n<td>Haemangioma\/ Haemangiosarcoma<\/td>\n<\/tr>\n<tr>\n<td>E Cadherine<\/td>\n<td>Epithelial and dendritic cells<\/td>\n<td>Histiocytic disorders \/ metastasis<\/td>\n<\/tr>\n<tr>\n<td>CD18 (canine)<\/td>\n<td>Integrine alfaXbeta2 \/ LFA1<\/td>\n<td>Leukocyte disorders (PMN, NK)<\/td>\n<\/tr>\n<tr>\n<td>CD18 (feline)<\/td>\n<td>Integrine alfaXbeta2 \/ LFA1<\/td>\n<td>Leukocyte disorders (PMN, NK)<\/td>\n<\/tr>\n<tr>\n<td>Lambda Chains<\/td>\n<td>B-Lymphocytes \/ Plasma cells<\/td>\n<td>B-cell Lymphoma \/ Myeloma<\/td>\n<\/tr>\n<tr>\n<td>CD11d<\/td>\n<td>Integrine alfaD<\/td>\n<td>Monocytic \/ Macrophage disorders<\/td>\n<\/tr>\n<tr>\n<td>Triptase<\/td>\n<td>Mast Cells<\/td>\n<td>Mast cell tumors<\/td>\n<\/tr>\n<tr>\n<td>Kappa Chains<\/td>\n<td>B-Lymphocytes \/ Plasma cells<\/td>\n<td>B-cell Lymphoma \/ Myeloma<\/td>\n<\/tr>\n<tr>\n<td>Myoglobin<\/td>\n<td>Striated muscle<\/td>\n<td>Rhabdomyosarcoma<\/td>\n<\/tr>\n<tr>\n<td>TNF alfa<\/td>\n<td>TNF alfa<\/td>\n<td>Proinflammatory cytokine<\/td>\n<\/tr>\n<tr>\n<td>IL6<\/td>\n<td>Interleuquine 6<\/td>\n<td>Proinflammatory cytokine<\/td>\n<\/tr>\n<tr>\n<td>CD117<\/td>\n<td>ckit<\/td>\n<td>Mast cell tumors<\/td>\n<\/tr>\n<tr>\n<td>Factor VIII<\/td>\n<td>Endothelial cells<\/td>\n<td>Hemangioma \/ Hemangiosarcoma<\/td>\n<\/tr>\n<tr>\n<td>MAC387<\/td>\n<td>Macrophages<\/td>\n<td>Myelomonocytic disorders<\/td>\n<\/tr>\n<tr>\n<td>Alpha Actin<\/td>\n<td>Smooth muscle<\/td>\n<td>Leiomyoma \/ Leiomyosarcoma \/ Myoepithelium<\/td>\n<\/tr>\n<tr>\n<td>Cytokeratins<\/td>\n<td>Epithelial cells<\/td>\n<td>Carcinoma<\/td>\n<\/tr>\n<tr>\n<td>Vimentin<\/td>\n<td>Mesenchymal cells<\/td>\n<td>Sarcoma<\/td>\n<\/tr>\n<tr>\n<td>Melan A<\/td>\n<td>Melanocytes<\/td>\n<td>Melanoma<\/td>\n<\/tr>\n<tr>\n<td>S100<\/td>\n<td>S100<\/td>\n<td>Periphereral Nerve Sheath Tumour \/ Melanoma<\/td>\n<\/tr>\n<tr>\n<td>GFAP<\/td>\n<td>GFAP<\/td>\n<td>Glial tumour<\/td>\n<\/tr>\n<tr>\n<td>Synaptophysin<\/td>\n<td>Synaptic vesicles<\/td>\n<td>Neuroendocrine Tumour<\/td>\n<\/tr>\n<tr>\n<td>Desmin<\/td>\n<td>Smooth and striated muscle<\/td>\n<td>Muscular Tumour<\/td>\n<\/tr>\n<tr>\n<td>NF<\/td>\n<td>Neurofilaments<\/td>\n<td>Neural \/ Endocrine Tumour<\/td>\n<\/tr>\n<tr>\n<td>PCNA<\/td>\n<td>S- and G1-Phase of cellular cycle<\/td>\n<td>Proliferation grade<\/td>\n<\/tr>\n<tr>\n<td>Ki67<\/td>\n<td>S-, G1- and G2-Phase of cellular cycle<\/td>\n<td>Proliferation grade<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table class=\"myTable\">\n<thead>\n<tr>\n<th>ANTIBODIES TO INFECTIOUS AGENTS<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Feline coronavirus (FcoV)<\/td>\n<\/tr>\n<tr>\n<td>Feline calicivirus \u00a0(FCV)<\/td>\n<\/tr>\n<tr>\n<td>Feline herpesvirus (FHV)<\/td>\n<\/tr>\n<tr>\n<td>Feline leukaemia virus (FeLV)<\/td>\n<\/tr>\n<tr>\n<td>Canine and feline parvovirus (FPV \/ CPV)<\/td>\n<\/tr>\n<tr>\n<td>Distemper (CDV)<\/td>\n<\/tr>\n<tr>\n<td>Borna disease virus (BDV)<\/td>\n<\/tr>\n<tr>\n<td>Toxoplasma gondii<\/td>\n<\/tr>\n<tr>\n<td>Neospora caninum<\/td>\n<\/tr>\n<tr>\n<td>Anaplasma phagocytophylum<\/td>\n<\/tr>\n<tr>\n<td>Helicobacter spp.<\/td>\n<\/tr>\n<tr>\n<td>Leishmania infantum<\/td>\n<\/tr>\n<tr>\n<td>Clamidia psitacii<\/td>\n<\/tr>\n<tr>\n<td>Coxiella burnetti<\/td>\n<\/tr>\n<tr>\n<td>Salmonella spp.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: #18acb4;\"><strong>MOLECULAR TESTS<\/strong><\/span><\/p>\n<p>Molecular tests (PCR) are used for the detection of mutations in Mast Cell neoplasia (MCT), to evaluate the response to treatment with Tyrosin Kinase inhibitors (TKI), as well as in lymphoproliferative disorders, allowing the distinction between lymphoid reactivity and lymphoma. In the case of lymphoma molecular tests also define the phenotype of the lymphoid neoplastic cells.<\/p>\n<p><span style=\"color: #18acb4;\"><strong>PROGNOSTIC TESTS<\/strong><\/span><\/p>\n<p>Prognostic Tests are used to evaluate the prognosis of specific neoplastic diseases in terms of aggressiveness, Median Survival Time (MST) and Disease-Free Interval (DFI). Markers of proliferation proteins and enzymes are used.<\/p>\n<table class=\"myTable\" style=\"height: 749px;\" width=\"670\">\n<thead>\n<tr>\n<th><\/th>\n<th><span style=\"color: #993366;\"><strong>TECHNIQUE<\/strong><\/span><\/th>\n<th style=\"text-align: center;\"><strong>APPLICABILITY<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>CANINE LYMPHOMA<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #993366;\">PCR<\/span><\/td>\n<td>Objective differentiation between reactive and neoplastic proliferation. Assessment of prognosis through phenotype<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>CANINE LYMPHOMA<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Subjective differentiation between reactive and neoplastic proliferation. Assessment of prognosis through phenotype<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>FELINE LYMPHOMA<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #993366;\">PCR<\/span><\/td>\n<td>Objective differentiation between reactive and neoplastic proliferation. Typing T \/ B<\/td>\n<\/tr>\n<tr>\n<td><span class=\"Apple-style-span\" style=\"color: #18acb4;\"><b>FELINE LYMPHOMA<\/b><\/span><\/td>\n<td style=\"text-align: center;\"><span class=\"Apple-style-span\" style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Subjective differentiation between reactive and neoplastic proliferation. Typing T \/ B<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>FELINE ALIMENTARY LYMPHOMA<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #993366;\">PCR<\/span><\/td>\n<td>Objective differentiation between lymphoplasmacytic enteritis (IBD) and alimentary lymphoma<\/td>\n<\/tr>\n<tr>\n<td><span class=\"Apple-style-span\" style=\"color: #18acb4;\"><b>FELINE ALIMENTARY LYMPHOMA<\/b><\/span><\/td>\n<td style=\"text-align: center;\"><span class=\"Apple-style-span\" style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Subjective differentiation between lymphoplasmacytic enteritis (IBD) and alimentary lymphoma<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>MASTOCITOMA CANINO<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #993366;\">PCR<\/span><\/td>\n<td>Objective assessment of mutations. Information about MST and DFI. Prognosis for mortality associated to tumor, recurrences and efficacy of conventional chemotherapy as well as TKI therapy<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>CANINE MAST CELL TUMOR<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span style=\"color: #993366;\">ICQ<\/span><\/td>\n<td>Subjective assessment of mutations. Information about MST and DFI<\/td>\n<\/tr>\n<tr>\n<td><span class=\"Apple-style-span\" style=\"color: #18acb4;\"><b>CANINE MAST CELL TUMOR<\/b><\/span><\/td>\n<td style=\"text-align: center;\"><span class=\"Apple-style-span\" style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Differentiation between &#8220;low grade&#8221; and &#8220;high grade&#8221; MCTs, and information about MST as well as possibility of recurrences and metastasis<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>CANINE AND FELINE MAMMARY CARCINOMA<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span class=\"Apple-style-span\" style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Information about MST<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>CANINE OSTEOSARCOMA\u00a0<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span class=\"Apple-style-span\" style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Information about DFI<\/td>\n<\/tr>\n<tr>\n<td><span style=\"color: #18acb4;\"><strong>CANINE MELANOMA<\/strong><\/span><\/td>\n<td style=\"text-align: center;\"><span class=\"Apple-style-span\" style=\"color: #993366;\">ICH<\/span><\/td>\n<td>Information about degree, MST and DFI<\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div><\/section>\n<div  class='hr av-av_hr-dcd2069817a0e3d34ae04cad8f3443c7 hr-default'><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<form action=\"https:\/\/histoweb.com\/en\/services\/special-techniques\/\" method=\"post\" data-fields-with-error=\"Found errors in the following field(s):\" class=\"avia_ajax_form av-form-labels-visible    \" data-avia-form-id=\"1\" data-avia-redirect='' ><h3 class=''>Should you have a comment or a question, please use the form below<\/h3><fieldset><p class='  first_form  form_element form_fullwidth' id='element_avia_1_1'><label for=\"avia_1_1\">Name <abbr class=\"required\" 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